Effects of encapsulation on Citrus reticulata Blanco somatic embryo conversion |
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Authors: | Germanà M. Antonietta Piccioni Emanuele Standardi Alvaro |
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Affiliation: | (1) Istituto di Ricerca per la Genetica degli Agrumi, CNR, Viale delle Scienze 11, 90128 Palermo, Italy;(2) Dipartimento di Aboricoltura e Protezione delle Piante, University of Perugia, Borgo XX Giugno 74, 06121 Perugia, Italy |
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Abstract: | Preliminary experiments on calcium-sodium alginate encapsulation of somatic embryos from anther culture were performed, in order to evaluate the effect of this technique on the recovery of plantlets, and the applicability of the synthetic seed technology to Citrus reticulata Blanco. Frequencies of conversion of somatic embryos on plant growth medium were 15.0%, 26.7% and 50.0%, respectively, when the somatic embryos were sown non-encapsulated, encapsulated with a hormone-less artificial endosperm or encapsulated with an artificial endosperm containing GA3. Encapsulation with GA3was also useful for storage of somatic embryos at 4 °C for one month; when sown on perlite medium, conversion frequencies were 25% with encapsulated somatic embryos against 5% with non-encapsulated embryos. Sowing on soil mix medium did not result in satisfactory conversion. This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | anther culture conversion somatic embryogenesis synthetic seed |
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