Artificial leucine rich repeats as new scaffolds for protein design |
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Authors: | Baabur-Cohen Hemda Dayalan Subashini Shumacher Inbal Cohen-Luria Rivka Ashkenasy Gonen |
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Institution: | Department of Chemistry, Ben Gurion University of the Negev, Beer Sheva, Israel |
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Abstract: | The leucine rich repeat (LRR) motif that participates in many biomolecular recognition events in cells was suggested as a general scaffold for producing artificial receptors. We describe here the design and first total chemical synthesis of small LRR proteins, and their structural analysis. When evaluating the tertiary structure as a function of different number of repeating units (1-3), we were able to find that the 3-repeats sequence, containing 90 amino acids, folds into the expected structure. |
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Keywords: | Leucine rich repeat proteins Native chemical ligation Peptides Protein design |
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