Production of an extracellular polysaccharide byAgrobacterium sp DS3 NRRL B-14297 isolated from soil |
| |
Authors: | Dr. C T Hou J A Ahlgren W Brown J J Nicholson |
| |
Affiliation: | (1) Oil Chemical Research, USDA, 1815 N University Street, 61604 Peoria, IL, USA;(2) Biopolymer Research Unit, National Center for Agricultural Utilization Research, ARS, USDA, 1815 N University Street, 61604 Peoria, IL, USA |
| |
Abstract: | A bacterium isolated from soil and identified asAgrobacterium sp produced a water-soluble extracellular polysaccharide capable of producing highly viscous solutions. Gas chromatographic analysis revealed a sugar composition of glucose, galactose and mannose in the molar ratio of 7.52.41, together with 3.7% (w/w) pyruvic acid. Methylation analyses showed the presence of (13)-, (14)- and (16)-linked glucose, (13)- and (14, 16)-linked galactose and a small portion of (13)-linked mannose residues. Succinic acid was not present. The molecular weight of the polysaccharide was estimated by light scattering to be 2×106 Da. The viscosity of solutions containing the polysaccharide remained constant from pH 3 to 11, and decreased by 50% when heated from 5 to 55°C. Maximum yield of the polysaccharide, 20 g L–1, was reached in 48 h at 30°C incubation. |
| |
Keywords: | extracellular polysaccharide Agrobacterium viscous polysaccharide |
本文献已被 SpringerLink 等数据库收录! |
|