Variation in epitopes of the B subunit of El Tor and classical biotype Vibrio cholerae O1 cholera toxin |
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| Authors: | M L Tamplin M K Ahmed R Jalali R R Colwell |
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| Institution: | Center of Marine Biotechnology, Maryland Biotechnology Institute, University of Maryland, Baltimore 21202. |
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| Abstract: | Variation in epitopes of the B subunit of cholera toxin (CT-B) produced by strains of El Tor and classical biotype Vibrio cholerae O1 was examined using monoclonal antibodies prepared to V. cholerae 569B CT. CT-B epitopes were markedly conserved for V. cholerae classical biotypes. In contrast, epitope variation was observed for El Tor biotypes, which produced both a classical-like CT-B and a unique CT-B lacking at least one epitope common to 569B CT-B. The missing epitope was located outside the GM1 ganglioside-binding site. From results of the study reported here, genetic divergence is exhibited in the El Tor biotype CT-B versus classical CT-B. Furthermore, at least five unique epitopes of V. cholerae 569B CT-B can be defined. |
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