Enhanced 2,3-butanediol production by <Emphasis Type="Italic">Klebsiella pneumoniae</Emphasis> SDM |
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Authors: | Cuiqing Ma Ailong Wang Jiayang Qin Lixiang Li Xulu Ai Tianyi Jiang Hongzhi Tang Ping Xu |
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Institution: | (1) State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People’s Republic of China;(2) Key Laboratory of Microbial Metabolism, Ministry of Education, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China;(3) School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China |
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Abstract: | Enhanced 2,3-butanediol (BD) production was carried out by Klebsiella pneumoniae SDM. The nutritional requirements for BD production by K. pneumoniae SDM were optimized statistically in shake flask fermentations. Corn steep liquor powder and (NH4)2HPO4 were identified as the most significant factors by the two-level Plackett–Burman design. Steepest ascent experiments were
applied to approach the optimal region of the two factors and a central composite design was employed to determine their optimal
levels. The optimal medium was used to perform fed-batch fermentations with K. pneumoniae SDM. BD production was then studied in a 5-l bioreactor applying different fed-batch strategies, including pulse fed batch,
constant feed rate fed batch, constant residual glucose concentration fed batch, and exponential fed batch. The maximum BD
concentration of 150 g/l at 38 h with a diol productivity of 4.21 g/l h was obtained by the constant residual glucose concentration
feeding strategy. To the best of our knowledge, these results were new records on BD fermentation.
Cuiqing Ma and Ailong Wang contributed equally to this work. |
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Keywords: | Klebsiella pneumoniae 2 3-Butanediol Medium optimization Fed batch |
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