Isoperoxidases as markers of somatic embryogenesis in carrot cell suspension cultures |
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Authors: | Morten Joersbo Jytte M. Andersen Finn T. Okkeis Ranganatha Rajagopal |
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Affiliation: | Biotechnology Section, A/S De Danske Sukkerfabrikker, DK-1001 Copenhagen K, Denmark;;Dept of Plant Physiology, The Royal Agricultural Univ., DK–1871 Copenhagen F, Denmark. |
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Abstract: | Growth, peroxidase activity and isoperoxidase pattern were studied during the growth cycle of 3 cell suspension lines of carrot ( Daucus carota L.), an embryogenic, a non-embryogenic and a habituated cell line. Isoelectric focusing of extracted proteins on agarose gels revealed the isoperoxidase pattern of the embryogenic line to include, among other differences, an isoperoxidase with a pl of pH 7.0 when grown under conditions stimulating embryogenesis. This isoperoxidase (P7.0: EC 1.11.1.7) was present between days 2 and 6 after subculturing, and this period correlates well with the early stages of somatic embryogenesis. This isoenzyme showed very low activity in the non-embryogenic and habituated cell suspension lines as well as in the embryogenic cell line in the presence of Daucus carota , 2,4–dichlorophenoxyacetic acid. P7.0 could probably be used as a biochemical marker of somatic embryogenesis. |
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Keywords: | Carrot cell suspension Daucus carota 2 4-dichlorophenoxyacetic acid isoperoxidases somatic embryogenesis |
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