Stress-induced expression of choline oxidase in potato plant chloroplasts confers enhanced tolerance to oxidative, salt, and drought stresses |
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Authors: | Raza Ahmad Myoung Duck Kim Kyung-Hwa Back Hee-Sik Kim Haeng-Soon Lee Suk-Yoon Kwon Norio Murata Won-Il Chung Sang-Soo Kwak |
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Institution: | (1) Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejon, 305-701, South Korea;(2) Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Eoeun-dong 52, Yuseong, Daejon, 305-806, South Korea;(3) Plant Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Eoeun-dong 52, Yuseong, Daejon, 305-806, South Korea;(4) National Institute for Basic Biology, Myodaiji, Okazaki 444-8585, Japan |
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Abstract: | Transgenic potato plants (Solanum tuberosum L. cv. Superior) with the ability to synthesize glycinebetaine (GB) in chloroplasts (referred to as SC plants) were developed
via the introduction of the bacterial choline oxidase (codA) gene under the control of an oxidative stress-inducible SWPA2 promoter. SC1 and SC2 plants were selected via the evaluation of methyl viologen (MV)-mediated oxidative stress tolerance,
using leaf discs for further characterization. The GB contents in the leaves of SC1 and SC2 plants following MV treatment
were found to be 0.9 and 1.43 μmol/g fresh weight by HPLC analysis, respectively. In addition to reduced membrane damage after
oxidative stress, the SC plants evidenced enhanced tolerance to NaCl and drought stress on the whole plant level. When the
SC plants were subjected to two weeks of 150 mM NaCl stress, the photosynthetic activity of the SC1 and SC2 plants was attenuated
by 38 and 27%, respectively, whereas that of non-transgenic (NT) plants was decreased by 58%. Under drought stress conditions,
the SC plants maintained higher water contents and accumulated higher levels of vegetative biomass than was observed in the
NT plants. These results indicate that stress-induced GB production in the chloroplasts of GB non-accumulating plants may
prove useful in the development of industrial transgenic plants with increased tolerance to a variety of environmental stresses
for sustainable agriculture applications. |
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Keywords: | Choline oxidase Environmental stress Glycinebetaine Metabolic engineering |
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