Site-specific restriction endonucleases in Bacillus licheniformis |
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Authors: | Carlo Parini M Grazia Fortina |
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Institution: | Dipartimento di Scienze e Tecnolagie Alimentari e Microbiologiche, Sezione di Microbiologia Industrielle, Unirersitàdegli Studi di Milano, Via Cetoria 2. 20133 Milan, Italy |
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Abstract: | Abstract We systematically studied site-specific restriction endonucleases in Bacillus licheniformis strains and detected endonuclease activity in 25 of 217 strains tested. Three different activities were obtained. One of these activities detected in 21 strains was the most representative within the species and produced a banding pattern, after digestion of A DNA, identical to that seen with Cla I. Two other strains isolated from soil samples from China and USA were found to produce a DNA-cleaving enzyme with the same recognition sequence as Bsa I. One producer strain, isolated from a Peruvian soil sample, showed to possess a mixture of two isoschizomers, Cla I and Bsa I. Finally, one strain produced an endonuclease activity, not previously described in B. licheniformis , that showed the same recognition sites as Bsu 361. |
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Keywords: | Bacillus licheniformis Type II restriction endonucleases BsaI ClaI Bsu361 isoschizomers |
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