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Cloth-based hybridization array system for detection of transgenic soy and corn by multiplex polymerase chain reaction
Authors:Burton W. Blais  Lucille M. Phillippe  Neil Vary
Affiliation:(1) Ottawa Laboratory (Carling), Canadian Food Inspection Agency, Ottawa, Canada, K1A 0C6
Abstract:A cloth-based hybridization array system (CHAS) was developed for detection of amplicons generated in a multiplex PCR targeting transgenic 35S and NOS sequences, as well as corn invertase and soy lectin genes. The CHAS provided confirmation of each amplicon on the basis of hybridization with specific capture probes, and enabled ease of discrimination of the multiplex PCR products by visualization of the amplicons on the array.
Keywords:array  cloth  corn  hybridization  soy  transgenic
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