Nutritional changes induce xylogenesis in callus ofHaplopappus gracilis (NUTT.) A. Gray |
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| Authors: | Wiesława Kutternozińska Lucyna Wajda W. Korohoda |
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| Affiliation: | (1) Laboratory of Cytophysiology, Department of Plant Physiology, Polish Academy of Sciences, 31-130 Kremerowska 2, Cracow;(2) Jan Zurzycki Institute of Molecular Biology, Jagiellonian University, 31-120 Aleje Mickiewicza 3, Cracow, Poland |
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| Abstract: | Xylogenesis was induced in cultures of calli ofHaplopappus gracilis (NUTT.) A. Gray by metabolic shocks brought about by changes in nutrition. Xylogenesis was observed to occur in response to three changes in callus nutrition, caused by the following modifications of Eriksson’s nutrient medium and culture conditions: i) omission of sucrose in the medium for 3 to 6 days and then transfer of the calli onto complete Eriksson’s medium; ii) replacement of sucrose by 0.36*mol l-1 xylose for the whole culture period; iii) omission of nitrogen sources(i.e. glycine and NH4NO3 omission and replacement of KNO3 with equimolar KCI) for the whole culture period. The induction of xylogenesis in response to nutritional stress inH. gracilis can be used as a suitable model system for research concerning plant cell differentiation. |
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