Human retinoblastoma: in vitro differentiation and immunoglobulin superfamily antigen modulation by retinoic acid |
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Authors: | R M Conway Michele C Madigan Nicholas J C King Francis A Billson Philip L Penfold |
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Institution: | (1) Save Sight and Eye Health Institute, Department of Clinical Ophthalmology, 1st Floor Sydney Eye Hospital, Sir John Young Cres, Woolloomooloo, NSW 2011 Australia, AU;(2) Department of Pathology, University of Sydney, Australia, AU |
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Abstract: | Suspension and attachment cultures of Y79 human retinoblastoma cells were treated with all-trans retinoic acid (RA) for up to 10 days to assess its effect on growth and cell-surface expression of immunoglobulin superfamily
antigens MHC class I and class II, ICAM-1, NCAM and Thy1. RA up to 10 μM induced growth inhibition, and marked morphological
differentiation with extension of prominent processes resembling neurites was seen in attachment cultures. However, above
10 μM RA produced extensive cell death. We also observed increased cell-surface expression of MHC class I, ICAM-1, NCAM and
Thy1 on Y79 cells treated with 10 μM over 10 days; constitutive MHC class II expression was not apparent, nor did RA treatment
appear to induce Y79 cells to express MHC class immunoreactivity. The up-modulation of cell-adhesion molecules (NCAM, ICAM-1
and Thy1) and immune recognition molecules (NCAM, ICAM-1 and MHC class I), associated with reduced growth and tumour cell
differentiation, suggests that RA may have a potential role in regulating the growth and development of retinoblastoma tumours.
Received: 29 August 1996 / Accepted: 16 January 1997 |
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Keywords: | Differentiation Immunoglobulin superfamily antigens Y79 retinoblastoma cell line All-trans retinoic acid Retina |
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