| miR-101抑制凋亡基因调控胰腺癌发生发展的初步研究 |
| |
| 引用本文： | 魏伟,唐翎,王琦,谭欣宇.miR-101抑制凋亡基因调控胰腺癌发生发展的初步研究[J].现代生物医学进展,2013,13(1):122-124. |
| |
| 作者姓名： | 魏伟 唐翎 王琦 谭欣宇 |
| |
| 作者单位： | 1. 中南大学湘雅医院普外科 湖南长沙410008
2. 中南大学湘雅医院药剂科 湖南长沙410008 |
| |
| 基金项目： | 湖南省科学技术厅项目(2010SK3099) |
| |
| 摘 要： | 目的：研究miR-101在胰腺癌组织中的表达水平及其作用机制。方法：采用实时定量PCR的方法分别检测36例胰腺癌手术切除标本及对应癌旁组织中miR-101的表达水平;运用生物信息学的方法预测其可能的靶基因;通过双荧光素酶报告基因系统验证miR-101与可能靶基因之间的关系。结果：胰腺癌组织中miR-101的表达水平较癌旁组织明显降低（P〈0.01）。生物信息学分析和双荧光素酶报告基因系统结果显示Mcl-1和Ezh2基因可能是miR-101的靶基因。结论：胰腺癌组织中miR-101表达降低,可能通过抑制胰腺癌细胞中Mcl-1和Ezh2基因的表达调控胰腺癌的发生发展。
|
| 关 键 词： | 胰腺癌 miR-101 生物信息学 Mcl-1 Ezh2 |
MiR-101 Regulated the Development of Pancreatic Cancer by Inhibiting the Expression of Apoptotic Genes |
| |
| WEI Wei,TANG Ling,△,WANG Qi,TAN Xin-yu.MiR-101 Regulated the Development of Pancreatic Cancer by Inhibiting the Expression of Apoptotic Genes[J].Progress in Modern Biomedicine,2013,13(1):122-124. |
| |
| Authors: | WEI Wei TANG Ling △ WANG Qi TAN Xin-yu |
| |
| Institution: | 1(1 Department of general surgery,Xiangya Hospital,Central South University,Changsha,410008,China; 2 Department of pharmacy,Xiangya Hospital,Central South University,Changsha,410008,China) |
| |
| Abstract: | Objective： To study the expression levels and the mechanism of miR-101 in the development of pancreatic cancer. Methods： Amount of 36 cases of pancreatic cancer surgical specimens and adjacent tissue were studied. The miR-101 expression level was determined by real-time PCR. The targeted genes ofmiR-101 were identified by bioinformatics algorithms and dual-luciferase reporter assay. Result： Real-time PCR assay showed that the expression level ofmiR-101 in pancreatic cancer tissue is significantly reduced com- pared to the adjacent tissue, and mcl-1 and ezh2 might be the potential targeted genes predicted by bioinformatics, which was confirmed by dual-luciferase reporter assay. Conclusion： miR-101 was down-expressed in pancreatic cancer, and might play important roles in the development of pancreatic cancer by repressing the expression of Mcl-1 and Ezh2. |
| |
| Keywords: | Pancreatic cancer miR-101 Bioinformatics Mcl-1 Ezh2 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
