纳米银-活性炭纤维敷料的体外抗菌实验研究

目的：观察比较纳米银-活性炭纤维敷料、纳米银敷料、碳纤维敷料和普通敷料对大肠杆菌、金黄色葡萄球菌和绿脓杆菌的抑菌效果,为临床创面合理应用敷料提供理论依据。方法：1）、抑菌圈法将敷料剪成直径为10mm的圆片,灭菌后贴于接菌的培养皿上,置37℃电热恒温培养箱培养16～24小时,游标卡尺测量抑菌圈大小。2）、振荡烧瓶实验称取被剪成10mm×10mm大小的敷料样品0.75g,灭菌后加于含70mL PBS和5mL浓度为1×104cfu/mL菌悬液的250 mL烧瓶,封口固定于振荡器上以300r/h分别振荡2min和1h,各取20μL样液作为振荡前和振荡后,均匀涂于MH培养皿,37℃电热恒温培养箱孵育16～24小时后计数,测定抑菌率。结果：与其他三组敷料相比,纳米银-活性炭纤维敷料的抑菌圈和抑菌率最大（P〈0.05）。结论：纳米银-活性炭纤维敷料具有较强的广谱抗菌作用,是目前临床上较为理想的创面抗感染敷料。

Experimental Study of in vitro Antibacterial Effect of nAgACF Dressing

Objective： To observe and compare the antibacterial effect： inhibition zone and inhibition rate of nAgACF dressing, nanosilver dressing, carbon fiber dressing and ordinary dressing to Escherichia coli, Staphylococcus anreus and Pseudomonas aeruginosa, to provide a theoretical basis for reasonable application of clinical wound dressing. Methods： 1 ）.Inhibition Zone The dressings were cut at diameter 10mm, affixed to the Petri dish inoculating bacteria after sterilization, and then be done to electric incubator at 37 ~C for 16 to 24 hours. The size of the zone of inhibition was measured by vernier caliper. 2）. The Shaking Flask Experiment Dressing sample were cut into size of 10mm x 10mm and weighed 0.75 g, after sterilizating then be added into an flask of 250mL containing 70 mL PBS and 5 mL of concentration of bacterial suspension at 1 x 104cfu/mL. Flask was sealed then fixed on the oscillator at 300r/h for 2min and 1 hour. Taking each of 20 ~L sample solution as the oscillation before and after, were evenly covered to the MH culture dish and then be done to electric incubator at 37 ~C for 16 to 24 hours then counting and calculating inhibition rate. Results： Compared with three other group of dressings, inhibition zone and inhibition rate of nAgACF dressings were biggest （P〈0.05）. Conclusions： nAgACF dressing has a strong broad-spectrum antibacterial effect and is a better ideal antibacterial dressing in clinical application.