Inhibition of EGF-Induced Signal Transduction by Microgravity Is Independent of EGF Receptor Redistribution in the Plasma Membrane of Human A431 Cells |
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Authors: | Philip J. Rijken Rolf P. De Groot Nico Van Belzen Siegfried W. De Laat Johannes Boonstra Arie J. Verkleij |
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Abstract: | Epidermal growth factor (EGF)-induced c-fos and c-jun expression is strongly suppressed in microgravity. We investigate here whether this is due to inhibition of processes occurring during the initiation of EGF-induced signal transduction. For this purpose, EGF-induced receptor clustering is used as a marker. The lateral distribution of EGF receptors is directly visualized at an ultrastructural level by the label-fracture method. Quantification of the receptor distributions shows that EGF-induced receptor redistribution is similar under normal and microgravity conditions. This suggests that microgravity influences EGF-induced signal transduction downstream of EGF binding and EGF receptor redistribution, but upstream of early gene expression in human A431 cells. |
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