Effect of superoxide and lipid peroxide on cytosolic free calcium concentration in cultured pig aortic endothelial cells |
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| Authors: | J Hirosumi Y Ouchi M Watanabe J Kusunoki T Nakamura H Orimo |
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| Affiliation: | Department of Geriatrics, Faculty of Medicine, University of Tokyo, Japan. |
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| Abstract: | The effect of reactive oxygen on cytosolic free calcium concentration [( Ca++]i) in pig aortic endothelial cells (ECs) was studied. Linoleate hydroperoxide (LHO) and superoxide radicals generated from xanthine with xanthine oxidase (X-XO) were used as sources of reactive oxygen. [Ca++]i in ECs was measured with quin 2 and the value for quiescent ECs was 112 +/- 11 nM. Both LHO and X-XO increased [Ca++]i in a dose-dependent manner without accompanying the significant cellular damage. Nifedipine suppressed the increase in [Ca++]i provoked by LHO and X-XO. Thus, the biological effects of reactive oxygen might be mediated, at least in part, by the activation of voltage-dependent calcium channels in ECs. |
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