Transport of phosphoenolpyruvate through red cell membrane in acidified isotonic sucrose medium. |
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Authors: | A Tomoda N Hamasaki S Minakami |
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Institution: | Department of Biochemistry Kyushu University School of Medicine Fukuoka 812, Japan |
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Abstract: | Organic phosphate compounds added exogenously are impermeable to the red cell membrane in physiological conditions. However, when the cells were incubated in an acidified iso-osmotic sucrose medium(pH 4.2), phosphoenolpyruvate passed freely the membrane, though other glycolytic intermediates and nucleotides failed to permeate the membrane. During incubation of the PEP loaded red cells,PEP was metabolized rapidly and almost one-to-one stoichiometry was observed in the relationship between 2,3DPG production and PEP depletion. |
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Keywords: | To whom reprint requests should be addressed |
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