Peptide and ester synthesis in organic solvents catalyzed by seryl proteases linked to alumina |
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| Authors: | M Pugnière A Skalli M A Coletti-Previero A Previero |
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| Institution: | Centre de Recherche, INSERM (U 58), Montpellier, France. |
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| Abstract: | Trypsin and alpha-chymotrypsin were immobilized to alumina-phosphocolamine complex, activated by glutaraldehyde. The immobilized enzymes show a great stability toward organic solvents miscible or immiscible with water. In the presence of a low concentration of water, the immobilized enzymes catalyzed transesterification reactions as well as peptide synthesis. The synthesized peptides were stable toward the immobilized enzymes. |
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| Keywords: | enzymatic transesterification peptide synthesis trypsin chymotrypsin |
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