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Biochemical and genetic heterogeneity of staphylococcal protein A |
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| Authors: | Junji Sakurada Li Zhijun Keiko Seki Miyo Murai Akemi Usui Mami Morihigashi Hiroko Jitsukawa Hee Kyung Seong Mitsuaki Mutou Shogo Masuda |
| Affiliation: | Department of Bacteriology, The Jikei University School of Medicine, Nishishinbashi 3-25-8, Minato-ku, Tokyo 105, Japan |
| Abstract: | Abstract We investigated the biochemical and genetic heterogeneity of protein A from Staphylococcus aureus . SpA genes ( spas ) of various strains were heterogeneous when detected as Dra I and Eco RV fragments of chromosomal DNA. Polymerase chain reaction using primers to detect DNA encoding the IgG-binding domains in spa revealed that they numbered between 2 and 5. Protein A from several S. aureus strains showed two types of reactivities to immunoglobulins in normal canine serum according to the number of active domains. |
| Keywords: | Staphylococcal protein A Heterogeneity of protein A Polymerase chain reaction |