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The identification and classification ofAcinetobacter strains exhibiting variations in phosphate accumulation by SDS-PAGE and numerical analysis
Authors:Mrs Marlene Bosch  Thomas E Cloete
Institution:(1) The Environmental Biotechnology Laboratory, Department of Microbiology and Plant Pathology, University of Pretoria, 0001 Pretoria, South Africa
Abstract:The limitation of phosphate concentrations in effluents is of international concern because of the risk of eutrophication.Acinetobacter spp. have often been isolated from activated sludge plants exhibiting enhanced phosphate removal. The ability to remove phosphate from mixed liquor byAcinetobacter isolates and reference strains was, therefore, determined. The ability to remove phosphate was found to vary among theAcinetobacter strains. The taxonomic relationships among these various strains were elucidated by SDS-PAGE and numerical analysis, and the correlation between their taxonomy and phosphate uptake ability was determined. The ability to remove large amounts of phosphate was found to be strain specific rather than species specific. The classification ofA. haemolyticus andA. baumannii as separate species is questioned.
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