| 一种从鸟类剥制标本提取DNA的改进方法 |
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| 引用本文： | 吴唤玲,韩德民,章敬旗,李进华.一种从鸟类剥制标本提取DNA的改进方法[J].动物学杂志,2007,42(1):84-88. |
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| 作者姓名： | 吴唤玲 韩德民 章敬旗 李进华 |
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| 作者单位： | 安徽大学生命科学学院,安徽省生态工程与生物技术重点实验室,合肥,230039 |
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| 基金项目： | 国家自然科学基金资助(No.30470258) |
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| 摘 要： | 应用非损伤性取样的方法,收集鸟类剥制标本的皮肤组织和羽毛,用无水乙醇、浸泡液预处理的方法抽提DNA,结果两者都可提取DNA供PCR扩增。将PCR产物经序列测定和比对分析,证明提取的DNA为目的DNA,表明本试验方法可行。鸟类剥制标本的皮肤组织和羽毛可以作为研究种群遗传学的资源。
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| 关 键 词： | 鸟类 剥制标本 DNA提取 勺鸡 皮肤组织 |
| 文章编号： | 0250-3263（2007）01-84-05 |
| 修稿时间： | 2006-03-20 |
An Improved Method for DNA Extraction from Avian Specimens |
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| WU HuanLing,HAN De-Min,ZHANG Jing-Qi,LI Jin-Hua.An Improved Method for DNA Extraction from Avian Specimens[J].Chinese Journal of Zoology,2007,42(1):84-88. |
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| Authors: | WU HuanLing HAN De-Min ZHANG Jing-Qi LI Jin-Hua |
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| Institution: | School of Life Science, Anhui University; The Key Laboratory of Eco-engineering and Biotechnique of Anhui Province, Hefei 230039, China |
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| Abstract: | Non-invasive sampling is a useful tool for genetic analysis of endangered species,but it is often inapplicable due to the low quality and quantity of DNA obtained.In order to explore a method for extracting DNA from specimen of bird,we tried to extract DNA from dried skin of birds.We extracted total DNA of the koklass specimens successfully.Compared with the existing DNA extracting methods,our method allows the use of minute amount of dried skin and the feather,and this method is easier,quicker and more economical.The specimens of the dried skin and feathers are proved to be a good source of DNA extraction for genetic analysis. |
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| Keywords: | Bird Specimen DNA extraction Koklass Skin |
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