Evolutionary conservation of kinetochore protein sequences in plants |
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Authors: | Rogier ten Hoopen Renate Manteuffel Jaroslav Dole?el Ludmilla Malysheva Ingo Schubert |
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Institution: | Institute of Plant Genetics and Crop Plant Research (IPK), 06466 Gatersleben, Germany. hoopen@ipk-gatersleben.de |
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Abstract: | The evolutionary conservation of structural/functional kinetochore proteins has been studied on isolated nuclei and pro-/metaphase chromosomes of mono- and dicot plants. The cross-reactivities of antibodies against human CENPC, CENPE and CENPF, and against maize CENPCa with the centromeric regions of mitotic chromosomes of Vicia faba and/or Hordeum vulgare are shown. Putative homologs of the kinetochore protein SKP1 (suppressor of kinetochore protein 1p of yeast) were found in both species and of CBF5p (centromere binding factor 5 of yeast) in barley. Antibodies against synthetic peptides derived from partial sequences encoding these proteins were produced and recognized the centromeric regions on mitotic chromosomes as detected by indirect immunofluorescence. |
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