Campylobacter protein oxidation influences epithelial cell invasion or intracellular survival as well as intestinal tract colonization in chickens |
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Authors: | A M ?asica A Wyszyńska K Szymanek P Majewski E K Jagusztyn-Krynicka |
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Institution: | (1) Produce Safety and Microbiology Research Unit, Agricultural Research Service U.S. Department of Agriculture, , 94710 Albany, CA, USA;(2) Department of Biotechnology, University of the Western Cape, Bellville, South Africa |
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Abstract: | The Dsb family of redox proteins catalyzes disulfide bond formation and isomerization. Since mutations indsb genes change the conformation and stability of many extracytoplasmic proteins, and since many virulence factors of pathogenic
bacteria are extracytoplasmic, inactivation ofdsb genes often results in pathogen attenuation. This study investigated the role of 2 membrane-bound oxidoreductases, DsbB and
DsbI, in theCampylobacter jejuni oxidative Dsb pathway.Campylobacter mutants, lacking DsbB or DsbI or both, were constructed by allelic replacement and used in the human intestinal epithelial
T84 cell line for the gentamicin protection assay (invasion assay) and chicken colonization experiments. InC. coli strain 23/1, the inactivation of thedsbB ordsbI gene separately did not significantly affect the colonization process. However, simultaneous disruption of both membrane-bound
oxidoreductase genes significantly decreased the strain’s ability to colonize chicken intestines. Moreover,C. jejuni strain 81–176 with mutateddsbB ordsbI genes showed reduced invasion/intracellular survival abilities. No cells of the double mutants (dsbB
−
dsbI
−) ofC. jejuni 81–176 were recovered from human cells after 3 h of invasion. |
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