The effect of cations on chymotrypsin from Aedes aegypti larvae |
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| Authors: | Y J Yang D M Davies |
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| Affiliation: | 1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province, PR China;2. Institute for Animal Hygiene, Animal Welfare and Farm Animal Behaviour, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany;1. College of Life Sciences, Gannan Normal University, Ganzhou City of Jiangxi Province, 341000, PR China;2. School of Information Engineering, Gannan Medical University, Ganzhou City of Jiangxi Province, 341000, PR China;1. Bacterial Protein Toxin Research Cluster, Institute of Molecular Biosciences, Mahidol University, Salaya Campus, Nakornpathom 73170, Thailand;2. Laboratory of Molecular Biophysics and Structural Biochemistry, Biophysics Institute for Research and Development (BIRD), Bangkok 10160, Thailand |
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| Abstract: | Several cations activated chymotrypsin from the larval mosquito, activation being proportional to the concentration of the salts. Ca2+ and Mg2+ were more effective than K+ and Na+. The pH optimum for the chymotrypsin was about 8·5 in the absence of cations, but greater than 10 in their presence. By dialysing the chymotrypsin preparation, about half the activity was lost but this was regained by adding Ca2+. Mosquito chymotrypsin appears to consist of at least two components, one being stable while, without cations, the other is unstable. Preliminary observations suggested that mosquito trypsin is inhibited by Ca2+. |
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