昆虫中肠Bt杀虫晶体蛋白毒素受体氨肽酶N的研究进展

鳞翅目昆虫中肠上皮细胞刷状缘膜(BBM)上的Bt杀虫晶体蛋白毒素受体氨肽酶N(APN)的结构和位点密度的改变是昆虫对Bt毒素的主要抗性机制之一，该文简要综述了APN受体的研究进展。每种昆虫中肠上皮细胞中有数种APNs，彼此间同源性较高，其中部分APNs为crylA家族毒素的功能性受体。不同种类昆虫的APNs受体，甚至同一种昆虫的不同类型APNs，其所结合的毒素种类可能不同。APNs决定该昆虫对crylA类毒素的敏感程度差异。有些抗性昆虫的APNs基因编码区发生了多个点突变。

Progress of studies on insect midgut aminopeptidase N serving as Bacillus thuringiensis insecticidal crystal protein toxin receptor

The changes of aminopeptidase N(APNs) in site concentration and structure are one of major resistance mechanisms of insects to Bacillus thuringiensis (Bt.) The authors reviewed the progress of research on APN receptor. A few types of APNs exist on the midgut brush border membrane of each species.They are highly homologous,and some of them serve as Bt Cry1A family toxin receptor.APNs maybe bind to different Bt toxins, and confer different level of susceptibility of Bt toxin to the insect.A few of mutations at the coding region of APN genes were found in some Bt resistant insects species.