Preliminary X-ray diffraction analysis of HhaII endonuclease-DNA cocrystals |
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Authors: | S Chandrasegaran H O Smith M L Amzel X Ysern |
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Affiliation: | Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205. |
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Abstract: | HhaII restriction endonuclease purified from an overproducing recombinant E. coli clone has been cocrystallized with a heptanucleotide duplex, d-GGAGTCC:GGACTCC. The cocrystals are monoclonic and belong to the space group C2. The unit cell dimensions are a = 199.0 +/- 1.0 A, b = 100.0 +/- 0.5 A, c = 80.3 +/- 0.4 A, and beta = 101.0 +/- 1.0 degrees. There appear to be two dimers per asymmetric unit and the crystals diffract to 4-A resolution. |
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Keywords: | protein-DNA interaction overproducer clone sequence-specific recognition |
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