The amino terminal sequence of cholera toxin subunits |
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| Authors: | J W Jacobs H D Niall G W Sharp |
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| Affiliation: | 1. Endocrine Unit Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114 USA;2. the Biochemical Pharmacology Unit Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02114 USA |
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| Abstract: | The -terminal amino acid sequence of Cholera toxin, molecular weight 84,000 daltons, has been established. A high sensitivity sequencing procedure, employing 35S-labelled phenylisothiocyanate as the coupling reagent in the automated Edman degradation was used. The toxin was found to consist of two polypeptide chains in the approximate molar ratio of 4:1. The amino-terminal twenty residues of each subunit will be reported here. |
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