Luciferase-YFP fusion tag with enhanced emission for single-cell luminescence imaging |
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Authors: | Hoshino Hideto Nakajima Yoshihiro Ohmiya Yoshihiro |
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Institution: | Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Osaka 563-8577, Japan. ah-hoshino@aist.go.jp |
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Abstract: | Taking advantage of the phenomenon of bioluminescence resonance energy transfer (BRET), we developed a bioluminescent probe composed of EYFP and Renilla reniformis luciferase (RLuc)--BRET-based autoilluminated fluorescent protein on EYFP (BAF-Y)--for near-real-time single-cell imaging. We show that BAF-Y exhibits enhanced RLuc luminescence intensity and appropriate subcellular distribution when it was fused to targeting-signal peptides or histone H2AX, thus allowing high spatial and temporal resolution microscopy of living cells. |
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