Deoxyribonuclease I (DNase I) |
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Authors: | Linardou H Deonarain M P Spooner R A Epenetos A A |
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Institution: | (1) Tumour Targeting Laboratory, Imperial Cancer Research Fund, Oncology Unit, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 OHS London, UK |
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Abstract: | A number of phosphodiesterases, some of which possess additional biological activities (e.g., antitumor, immunosupressive,
and so on), have been considered for use in targeted tumor therapy. We propose Deoxyribonuclease I (DNase I), a compact, monomeric
enzyme, as a very attractive candidate for targeting to tumor cells. Only a small amount of enzyme targeted to a cell needs
to enter the nucleus in order to degrade the chromosomal DNA, making a cell incapable of further replication. We describe
preliminary data on the construction of a potent single-chain antibody (scFv) immunotoxin based on bovine pancreatic DNase
I. The use of a mammalian enzyme should be much less toxic and less immunogenic than current immunotoxins and may expand the
current limits of immunotoxin therapy. |
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Keywords: | Bovine pancreatic deoxyribonuclease I single-chain antibody human placental alkaline phosphatase mammalian enzyme immunotoxin |
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