A New Mesorhizobium loti HAMBI 1129 Phage Isolated from Polish Soil |
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Authors: | Anna Turska-Szewczuk Ryszard Russa |
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Institution: | (1) Department of General Microbiology Maria Curie-Skłodowska University, 19 Akademicka St., 20-033 Lublin, Poland , PL |
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Abstract: | Phage A1 isolated from the rhizosphere of Lotus corniculatus was studied. It had a very narrow host range, as it was active only against Mesorhizobium loti HAMBI 1129. Phage A1 was classified as belonging to C Bradley's group bacteriophages. The latent period of A1 was 120–130
min and a burst size 13–17 particles per cell. The nature of the phage receptor was examined. Lipopolysaccharide from the
phage-sensitive strain inactivated phage A1 in contrast to LPS from the phage-resistant bacteria. Purified LPS obtained from
M. loti HAMBI 1129 had a high receptor activity with PhI50 value of 0.025 μg/ml.
Received: 30 July 1999 / Accepted: 15 November 1999 |
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