二氧化硅及柠檬酸铝对红细胞膜结合水影响的研究

本研究应用等温吸附法和付里埃变换红外光谱技术测定了二氧化硅及柠檬酸铝对红细胞膜结合水的不同影响。结果为,二氧化硅明显降低细胞膜水化度,使膜结合水的ν_OH峰位显著红移,表明其可导致细胞膜脱水。而柠檬酸铝对二氧化硅的这一作用有明显的拮抗效应,即通过提高细胞膜的水化度,可维持细胞膜的正常“水结构”。此外,本文还论讨了二氧化硅诱发细胞膜的脱水作用对细胞中毒的意义,以及柠檬酸铝拮抗作用的机理。

THE INVESTIGATION OF THE EFFECTS OF SILICA AND ALUMINIUM CITRATE ON ERYTHROCYTE MEMBRANE-BOUND WATER

In the present study, the different effects of silica and aluminium citrate on erythrocyte membrane-bound water have been determined, using method of sorption isotherm and technique of FT-IR spectroscopy. Results show that silica reduces markedly membrane hydration with the red shift of the streching vibration band of water (voH) . This fact indicates that silica causes dehydration of cell membrane. The antagonistic effect of aluminium citrate on dehydration caused by silica has been also demonstrated. It increases membrane hydration and maintains the "water structure" at the normal level. In addition, the role of dehydration induced by silica in its Cytotoxity and the mechanism of antagonistic effect of aluminium citrate on silica have also been discussed.