A packed-bed fungal bioreactor for the continuous decolourisation of azo-dyes (Orange II) |
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Authors: | Mielgo I Moreira M T Feijoo G Lema J M |
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Institution: | Department of Chemical Engineering, Institute of Technology, University of Santiago de Compostela, E-15706 Santiago de Compostela, Gazila, Spain |
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Abstract: | The degradation of an azo dye, Orange II, by immobilised Phanerochaete chrysosporium in a continuous packed bed bioreactor for periods longer than 30 days has been carried out. Nearly complete decolourisation (>95%) was achieved when working at a high dye load rate of 0.2 g l?1 d?1, a temperature of 37 °C, a hydraulic retention time (HRT) of 24 h and applying oxygen gas in a pulsed flow. These conditions allowed Manganese peroxidase (MnP) production and the subsequently Orange II decolourisation. A correlation between residual MnP activity in the effluent and decolourisation was established. Apparently, for decolourisation to be effective, a minimum MnP activity was required, no substantial increase in efficiency at MnP activities higher than 10 U 1 ?1 was observed. The treatment caused, the breakdown of the chromophoric group as well as the cleavage of the aromatic ring. |
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Keywords: | Decolourisation Azo dye Packed bed bioreactor Immobilised Phanerochaete chrysosporium |
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