A Preliminary Linkage Map of the Tick, Ixodes scapularis |
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| Authors: | Amy J. Ullmann Joseph Piesman M.C. Dolan William C. Black IV |
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| Affiliation: | (1) P.O. Box 2087, Rampart Road, Foothills Campus, Fort Collins, CO 80522, USA;(2) Department of Microbiology, Colorado State University, Fort Collins, CO 80523-1677, USA |
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| Abstract: | A linkage map of the Ixodes scapularis genome was constructed based upon segregation amongst 127 loci. These included 84 random amplified polymorphic DNA (RAPD) markers, 32 Sequence-Tagged RAPD (STAR) markers, 5 cDNAs, and 5 microsatellites in 232 F1 intercross progeny from a single, field-collected P1 female. A preliminary linkage map of 616 cM was generated across 14 linkage groups with one marker every 10.8 cM. Assuming a genome size of ∼109 bp, the relationship of physical to genetic distance is ∼300 kb/cM in the I. scapularis genome. This revised version was published online in July 2006 with corrections to the Cover Date. |
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| Keywords: | Ixodes scapularis linkage map microsatellites RAPD-SSCP STARs cDNA-SSCP |
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