Haploid plants from in vitro anther culture of the leguminous tree,Peltophorum pterocarpum (DC) K. Hayne (Copper pod) |
| |
Authors: | P V Lakshmana Rao N De Deepesh |
| |
Institution: | (1) Applied Botany Section, Agricultural Engineering Department, Indian Institute of Technology, 721 302 Kharagpur, India;(2) Present address: Defence Research Laboratory, P.O. Box No. 2, 784 001 Tezpur, Assam, India |
| |
Abstract: | The development of haploid callus, embryos and plantlets from cultured anthers and the various factors affecting androgenesis in Peltophorum pterocarpum (Copper pod), a tropical legume tree is reported. A pretreatment of flower buds at moderate temperature of 14°C for 8 days was most effective for callus production. The colour of the anther was found to be a reliable and efficient indicator for identification of suitable stage of anther for culture. The frequency of anthers which produced callus and shoots was highest when anthers were cultured at mid or late-uninucleate stage. A high sucrose concentration of 10% is a specific media requirement for androgenesis. The haploid nature of the embryos, callus and regenerated plants (n=14) were confirmed by chromosome count.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- KN
kinetin
- NAA
naphthaleneacetic acid
- BAP
bezylaminopurine |
| |
Keywords: | Peltophorum pterocarpum anther culture pollen plants haploids tree legume |
本文献已被 SpringerLink 等数据库收录! |
|