cDNA cloning and expression analysis of new members of the mammalian F-box protein family |
| |
Authors: | Ilyin G P Rialland M Pigeon C Guguen-Guillouzo C |
| |
Affiliation: | INSERM U522, H?pital Pontchaillou, Rennes, France. Guennadi.Iline@rennes.inserm.fr |
| |
Abstract: | F-box proteins are critical components of the SCF ubiquitin-protein ligase complex and are involved in substrate recognition and recruitment for ubiquitination and consequent degradation by the proteasome. We have isolated cDNAs encoding a further 10 mammalian F-box proteins. Five of them (FBL3 to FBL7) share structural similarities with Skp2 and contain C-terminal leucine-rich repeats. The other 5 proteins have different putative protein-protein interaction motifs. Specifically, FBS and FBWD4 proteins contain Sec7 and WD40-repeat domains, respectively. The C-terminal region of FBA shares similarity with bacterial protein ApaG while FBG2 shows homology with the F-box protein NFB42. The marked differences in F-box gene expression in human tissues suggest their distinct role in ubiquitin-dependent protein degradation. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|