Gene-based anchoring of the rat genetic linkage and cytogenetic maps: new regional localizations, orientation of the linkage groups, and insights into mammalian chromosome evolution |
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Authors: | Claude Szpirer Josiane Szpirer Pascale Van Vooren Fadel Tissir Jason S Simon George Koike Howard J Jacob Eric S Lander Khalil Helou Karin Klinga-Levan Göran Levan |
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Institution: | Département de Biologie Moléculaire, Université Libre de Bruxelles, Rue des Chevaux, 67, B-1640 Rhode-Saint-Genèse, Belgium, BE Cardiovascular Research Center, Massachusetts General Hospital and Harvard Medical School, Charlestown Massachusetts 02129, USA, US Medical College of Wisconsin, Department of Physiology, Milwaukee, Wisconsin 53226-0509, USA, US Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02139 USA, US CMB-Genetics, G?teborg University, S-405 30 Gothenburg, Sweden, SE
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Abstract: | In order to generate anchor points connecting the rat cytogenetic and genetic maps, the cytogenetic position of 62 rat markers
(including 55 genes) already localized genetically was determined by fluorescence in situ hybridization. Whenever possible,
markers located near one end of the linkage groups were included. These new localizations allowed us to unambiguously orient
the 20 autosomal and the X chromosome linkage groups. The position of the centromere in the linkage map could also be determined
in the case of several metacentric chromosomes. In addition, the regional localization of 15 other rat genes was determined.
These new data bring useful information with respect to comparative mapping with the mouse and the human and to mammalian
evolution. They illustrate, for instance, that groups of genes can remain syntenic during mammalian evolution while being
subjected to intrachromosomal rearrangements in some lineages (synteny is conserved while gene order is not). This analysis
also disclosed cases of synteny conservation in one the two rodent species and the human, while the synteny is split in the
other rodent species: such configurations are likely examples of lineage-specific interchromosomal rearrangements associated
with speciation.
Received: 20 April 1998 / Accepted: 26 May 1998 |
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