Structural elucidation of a 3-O-methyl-D-galactose-containing neutral polysaccharide from the fruiting bodies of Phellinus igniarius |
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Authors: | Yang Yan Zhang Jingsong Liu Yanfang Tang Qingjiu Zhao Zigao Xia Wenshui |
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Institution: | Key Laboratory of Food Science and Safety, Ministry of Education, Southern Yangtze University, Wuxi 214060, Jiangsu, China. yangyan9200@yahoo.com.cn |
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Abstract: | PIP60-1, a novel heteropolysaccharide isolated from fruiting bodies of the medicinal fungus, Phellinus igniarius, has a molecular weight of 1.71 x 10(4)Da and is composed of L-fucose, D-glucose, D-mannose, D-galactose and 3-O-Me-D-galactose in a ratio of 1:1:1:2:1. A structural investigation of PIP60-1 carried out using sugar and methylation analyses, combined with (1)H and (13)C NMR spectroscopy, including COSY, TOCSY, NOESY, HSQC and HMBC experiments, established the repeating unit of the polysaccharide as the following: structure: see text] |
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Keywords: | Phellinus igniarius Polysaccharide Structural analysis NMR spectroscopy |
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