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Construction of shuttle vectors useful for transforming Clostridium acetobutylicum
Authors:N Truffaut  J Hubert  G Reysset
Institution:International Institute of Genetics and Biophysics, CNR, Naples, Italy.
Abstract:The symbiotic plasmid (pSym) DNA present in bacteroids of strain RCR1001 of Rhizobium leguminosarum biovar viceae has been compared qualitatively and quantitatively with that present in free living bacteria by hybridization experiments with appropriate probes. A decrease in the relative amount of pSym DNA was observed in bacteroids as compared to bacteria. No rearrangements of the symbiotically expressed pSym borne genes were detected in bacteroids.
Keywords:Clostridium acetobutylicum            Vector  Transformation
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