Agrobacterium-mediated transient transformation of annatto (Bixa orellana) hypocotyls with the GUS reporter gene |
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| Authors: | Zaldívar-Cruz JM Ballina-Gómez H Guerrero-Rodríguez C Avilés-Berzunza E Godoy-Hernández GC |
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| Institution: | (1) Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigación Científica de Yucatán, A.C. C.43, No. 130, Chuburná de Hidalgo, 97200 Mérida, Yucatán, Mexico |
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| Abstract: | Hypocotyls from annatto seedlings, were inoculated with Agrobacterium tumefaciens harboring a binary vector, pBI.121 or pCAMBIA2301, containing the  -glucuronidase (gus) gene. Histochemical GUS assay of infected hypocotyls from two annatto varieties showed transient gus gene expression between 3 and 12 days after inoculation.These authors contributed equally to this work.) |
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| Keywords: | genetic transformation  -glucuronidase" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-glucuronidase neomycin phosphotransferase II |
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