Specific lipid requirements of membrane proteins—a putative bottleneck in heterologous expression |
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Authors: | M Opekarová |
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Institution: | a Institute of Microbiology, Czech Academy of Sciences, 142 20 Prague 4, Czech Republic b Universität Regensburg, Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universitätsstrasse 31, 93053 Regensburg, Germany |
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Abstract: | Membrane proteins are mostly protein-lipid complexes. For more than 30 examples of membrane proteins from prokaryotes, yeast, plant and mammals, the importance of phospolipids and sterols for optimal activity is documented. All crystallized membrane protein complexes show defined lipid-protein contacts. In addition, lipid requirements may also be transitory and necessary only for correct folding and intercellular transport. With respect to specific lipid requiremnts of membrane proteins, the phospholipid and glycolipid as well as the sterol content of the host cell chosen for heterologous expression should be carefully considered. The lipid composition of bacteria, archaea, yeasts, insects,Xenopus oocytes, and typical plant and mamalian cells are given in this review. A few examples of heterologous expression of membrane proteins, where problems of speific lipid requirements have been noticed or should be thought of, have been chosen. |
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Keywords: | Lipid requirement Membrane protein Heterologous expression |
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