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Identification of novel proteolytic forms of osteocalcin in human urine |
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| Authors: | Ivaska Kaisa K Hellman Jukka Likojärvi Johanna Käkönen Sanna-Maria Gerdhem Paul Akesson Kristina Obrant Karl J Pettersson Kim Väänänen H Kalervo |
| Institution: | a Department of Anatomy, Institute of Biomedicine, University of Turku, Kiinamyllynkatu 10, FIN-20520 Turku, Finland b Department of Biotechnology, University of Turku, Tykistökatu 6A, FIN-20520 Turku, Finland c Department of Orthopaedics, Malmö University Hospital, SE-20502 Malmö, Sweden |
| Abstract: | In this study, we report the isolation and characterization of osteocalcin in human urine using mass spectrometry and N-terminal sequencing. Multiple proteolytic forms of osteocalcin were found, which consisted of 16-27 residues from the middle region of the molecule. Several fragments had residue Gly7 at the N-terminus and the most predominant was fragment 7-31. Additional fragments starting from residue Asp14 were detected in the samples of children and young adults. Immunochemical detection of urine osteocalcin fragments had a statistically significant negative correlation to bone mineral density in evaluation of urine samples from 75-year-old women. Thus, the measurement of osteocalcin fragments in urine may have potential applications in diagnostics related to disorders of bone metabolism. |
| Keywords: | Bone Urine Osteocalcin Turnover Marker Fragment Mass spectrometry Bone mineral density |
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