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Photosystem I trimers from Synechocystis PCC 6803 lacking the PsaF and PsaJ subunits bind an IsiA ring of 17 units |
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| Authors: | Roman Kou?il Sandrine D'Haene Wilko Keegstra Jan P Dekker |
| Institution: | a Department of Biophysical Chemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands b Aquatic Microbiology, Institute for Biodiversity and Ecosystem Dynamics, Faculty of Science, University of Amsterdam, Nieuwe Achtergracht 127, 1018 WS Amsterdam, The Netherlands c Faculty of Sciences, Department of Physics and Astronomy, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands |
| Abstract: | We report a structural characterization by electron microscopy and image analysis of a supramolecular complex consisting of Photosystem I (PSI) and the chlorophyll-binding protein IsiA from a mutant of the cyanobacterium Synechocystis PCC 6803 lacking the PsaF and PsaJ subunits. The circular complex consists of a central PSI trimer surrounded by a ring of 17 IsiA units, one less than in the wild-type supercomplex. We conclude that PsaF and PsaJ are not obligatory for the binding of the IsiA ring, and that the size of the PSI complex determines the number of IsiA units in the ring. The resulting number of 17 copies implies that each PSI monomer has a different association to the IsiA ring. |
| Keywords: | Photosystem I IsiA Electron microscopy |
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