a Departement Chemie, Katholieke Universiteit Leuven, Celestijnenlaan 200 D, B-3001 Leuven, Belgium b Universität Leipzig, Institut für Experimentelle Physik I, Linnéstr. 5, D-04103 Leipzig, Germany
Abstract:
Dehydration reduces the main phase transition pressure of phospholipids. An analysis based on the Gibbs-Duhem equation shows how the shift of the transition pressure is correlated to the hydration pressure.By using Fourier transform infrared (FT-IR) spectroscopy we determined the hydration-dependent phase transition pressure. The application of our new approach gives hydration pressure values which agree with the values obtained with the osmotic stress method.