Physiological role of d-amino acid-N-acetyltransferase of Saccharomyces cerevisiae: detoxification of d-amino acids |
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Authors: | Geok-Yong Yow Takuma Uo Tohru Yoshimura Nobuyoshi Esaki |
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Institution: | (1) Institute for Chemical Research, Kyoto University, Gokasho, Uji, 611-0011 Kyoto-fu, Japan;(2) Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, 464-8601 Nagoya-City, Aichi, Japan |
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Abstract: | Saccharomyces cerevisiae is sensitive to d-amino acids: those corresponding to almost all proteinous l-amino acids inhibit the growth of yeast even at low concentrations (e.g. 0.1 mM). We have determined that d-amino acid-N-acetyltransferase (DNT) of the yeast is involved in the detoxification of d-amino acids on the basis of the following findings. When the DNT gene was disrupted, the resulting mutant was far less tolerant
to d-amino acids than the wild type. However, when the gene was overexpressed with a vector plasmid p426Gal1 in the wild type
or the mutant S. cerevisiae as a host, the recombinant yeast, which was found to show more than 100 times higher DNT activity than the wild type, was
much more tolerant to d-amino acids than the wild type. We further confirmed that, upon cultivation with d-phenylalanine, N-acetyl-d-phenylalanine was accumulated in the culture but not in the wild type and hpa3Δ cells overproducing DNT cells. Thus, d-amino acids are toxic to S. cerevisiae but are detoxified with DNT by N-acetylation preceding removal from yeast cells. |
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Keywords: | Saccharomyces cerevisiae d-amino acids" target="_blank">d-amino acids Toxicity d-amino acid-N-acetyltransferase" target="_blank">d-amino acid-N-acetyltransferase |
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