| 乳鼠感染流行性出血热病毒的研究 |
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| 引用本文： | 李德荣,洪松芳,宋光昌,李钟铎,何锦芳,傅鸿鸣,潘春华,王宏霞,李予川,王叙甫,朱关福. 乳鼠感染流行性出血热病毒的研究[J]. 病毒学报, 1985, 0(4) |
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| 作者姓名： | 李德荣 洪松芳 宋光昌 李钟铎 何锦芳 傅鸿鸣 潘春华 王宏霞 李予川 王叙甫 朱关福 |
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| 作者单位： | 军事医学科学院微生物流行病研究所 北京(李德荣，洪松芳，宋光昌，李钟铎，何锦芳，傅鸿鸣，潘春华，王宏霞，李予川，王叙甫)，军事医学科学院微生物流行病研究所 北京(朱关福) |
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| 摘 要： | 用获自病人的流行性出血热(EHF)病毒H8205株感染Vero-E6细胞的培养液,感染1日龄小白鼠,自第3代起,感染鼠出现规律发病,潜伏期13～18天,个体瘦小,后肢麻痹,最后死亡。病理检查感染鼠脑有典型病毒性脑炎改变,免疫荧光及酶标抗体染色证实脏器中存在特异性抗原,血内有特异性抗体,感染鼠的血,尿及脏器悬液经Vero-E6细胞培养出病毒。电镜检查脑神经原细胞高尔基氏器内有病毒颗粒。实验结果说明1日龄小白鼠对流行性出血热病毒是敏感的,可以作为该病毒的实验动物模型。
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| 关 键 词： | 流行性出血热病毒 乳鼠模型 特异抗原抗体检出 电镜检查 |
EXPERIMENTAL INFECTION OF SUCKLING MICE WITH EPIDEMIC HEMORRHAGIC FEVER VIRUS |
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| Li Derong Hong Songfang Song Guangchang Li ZhongduoHe Jinfang,Fu Hongming Pan Chunhua Wang HongxiaLi Yuchuan Wang Xufu Zhu Guanfu. EXPERIMENTAL INFECTION OF SUCKLING MICE WITH EPIDEMIC HEMORRHAGIC FEVER VIRUS[J]. Chinese journal of virology, 1985, 0(4) |
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| Authors: | Li Derong Hong Songfang Song Guangchang Li ZhongduoHe Jinfang Fu Hongming Pan Chunhua Wang HongxiaLi Yuchuan Wang Xufu Zhu Guanfu |
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| Abstract: | Suckling mice inoculated with EHF virus isolated from patients regularly showed signs of infection such as growth retardation and paralysis of the hind legs. Microscopic observations revealed various patho-histologicalchangs in many organs, especially encephalitic lesion in the cortex and hippocampus. By immunofluorescent and immunoperoxidase staining, infected cells containing EHF viral antigen were found in the brarn and other organs. Specific antibodies ( IgM, IgG ) were detected in the sera of infected mice. EHF virus could be isolated from brain, blood and urine collected from 3 to 14 days after infection. EHF viral particles could be found in-dilated Golgi vesicles. These results showed that the mouse is sensitive to EHF virus and could serve as an experimental animal model for EHF. |
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| Keywords: | Epidemic hemorrhagic fever Suckling mouse Electron microscopy |
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