Internal pH of human neutrophil lysosomes |
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| Authors: | B Styrt M S Klempner |
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| Institution: | Institute of Crystallography and Institute of Molecular Biology, USSR Academy of Sciences, Moscow, USSR |
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| Abstract: | We focus this report on the relationship between signal II fast and slow during a flash sequence for Tris-washed chloroplasts at different pH-values. The pH influences both the redox state and spectral form of signal II slow in dark-adapted chloroplasts. At pH 6.0, signal II slow is oxidized and does not influence the kinetics of signal II fast equally formed on each flash. At pH 8.5, signal II slow in mainly reduced in the dark and the first flash produces signal II slow and no signal fast. Signal II fast appears on the following flashes only. Signals II fast and slow are connected to the same center and signal II fast is observed only if signal II slow is oxidized. |
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| Keywords: | cAATase cytosolic aspartate aminotransferase mAATase mitochondrial aspartate aminotransferase PLP pyridoxal-5′-phosphate MMA methylmercuriacetate DTNB 5 5'-dithiobis-(2-nitrobenzoate) |
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