Molecular mechanisms involved in the production of chromosomal aberrations |
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| Authors: | A T Natarajan G Obe |
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| Institution: | (1) Department of Radiation Genetics and Chemical Mutagenesis, University of Leiden, Sylvius Laboratories, Wassenaarseweg 72, 2333 AL Leiden, The Netherlands;(2) Interuniversity Institute for Radiopathology and Radiation Protection, J.A. Cohen Institute, Rijnsburgerweg 86, 2333 AD Leiden, The Netherlands;(3) Institute of Genetics, Free University of Berlin, Arnimallee 5-7, D-1000 Berlin 33, Germany |
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| Abstract: | Chinese hamster ovary cells (CHO cells) and mouse fibroblasts (PG 19) were permeabilized with inactivated Sendai virus, treated with different types of restriction endonucleases (Eco RV, Pvu II, Bam HI, Sma I, Asu III, Nun II), and studied for the occurrence of chromosomal aberrations at different times following treatment. The pattern of chromosomal aberrations observed was similar to that induced by ionizing radiations. Restriction endonucleases that induce blunt double-strand breaks (Eco RV, Pvu II) were more efficient in inducing chromosomal aberrations than those that induce breaks with cohesive ends (Bam HI, Nun II, Asu III). Ring types were very frequent among the aberrations induced by restriction enzymes. Cytosine arabinoside, an inhibitor of DNA repair, was found to increase the frequencies of aberrations induced by restriction enzymes, indicating its effect on ligation of double-strand breaks. The relevance of these results to the understanding of the mechanisms of chromosomal aberration formation following treatment with ionizing radiations is discussed. |
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