Cytologically based physical maps of the group-2 chromosomes of wheat |
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Authors: | D. E. Delaney S. Nasuda T. R. Endo B. S. Gill S. H. Hulbert |
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Affiliation: | (1) Department of Plant Pathology, Kansas State University, 66506 Manhattan, Kansas, USA;(2) Laboratory of Genetics, Faculty of Agriculture, Kyoto University, 606 Kyoto, Japan |
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Abstract: | We have constructed cytologically based physical maps (CBPMs), depicting the chromosomal distribution of RFLP markers, of the group-2 chromosomes of common wheat (Triticum aestivum L. em Thell). Twenty-one homozygous deletion lines for 2A, 2B, and 2D were used to allocate RFLP loci to 19 deletion-interval regions. A consensus CBPM was colinearily aligned with a consensus genetic map of group-2 chromosomes. The comparison revealed greater frequency of recombination in the distal regions. Several molecularly tagged chromosome regions were identified which may be within the resolving power of pulsed-field gel electrophoresis. The CBPMs show that the available probes completely mark the group-2 chromosomes, and landmark loci for sub-arm regions were identified for targeted-mapping. |
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Keywords: | RFLPs Deletion mapping Recombination Cereals |
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