Preparative Electrophoresis of Isotopically Labeled L-Cell Interferons |
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Authors: | Daniel Stan?ek and Kurt Paucker |
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Abstract: | The preparative method of polyacrylamide gel electrophoresis was adapted for purification and characterization of isotopically labeled L-cell interferons. Re-covery of interferon activity was quantitative, and purification and resolution were comparable to those obtained by analytical polyacrylamide gel electrophoresis. Ultimate specific activities attainable ranged from 2 x 10(6) to 3 x 10(6) international units per mg of protein. |
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