An <Emphasis Type="Italic">in vitro</Emphasis> microbial model associated with sucrose to produce dentin caries lesions |
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Authors: | Carolina Steiner-Oliveira Lidiany Karla Azevedo Rodrigues Iriana Carla Junqueira Zanin Carolina Lima de Carvalho Regianne Umeko Kamiya Anderson Takeo Hara Marinês Nobre-dos-Santos |
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Institution: | 1.Department of Pediatric Dentistry, Piracicaba Dental School,University of Campinas,Piracicaba, SP,Brazil;2.Faculty of Pharmacy, Dentistry and Nursing,Federal University of Ceará,Fortaleza, CE,Brazil;3.Faculty of Dentistry,Federal University of Ceará,Sobral, CE,Brazil;4.Department of Oral Diagnosis, Microbiology and Immunology Laboratories — Piracicaba Dental School,University of Campinas,Piracicaba,Brazil;5.Oral Health Research Institute,Indiana University School of Dentistry,Indianapolis,USA |
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Abstract: | The complexity of the oral environment and ethical issues have prompted the development of an in vitro bacterial model to evaluate the effect of frequency of sucrose exposure on dentin caries formation, biofilm composition,
and pH changes. In the experiment, dentin specimens (n=45) were randomly divided into four groups: control (C), negative control
(0S), 3S (three sucrose baths), and 6S (six sucrose baths). The specimens then were inoculated with Streptococcus mutans and treated according to the protocol described below. Dentin demineralization and lesion depth were assessed by transverse
microradiography. Extracellular polysaccharides that formed in the biofilm were analyzed and counts of microorganisms in the
carious dentin were measured. After a 7-day period of growth, the biofilm pH was assessed before and after sucrose baths (n=5).
The addition of sucrose led to dentin caries development regardless of the number of sucrose baths performed. The number of
colony forming units (cfu) from the carious dentin did not differ among the treatment groups, though the extracellular polysaccharides
from both 3S and 6S differed from 0S. The pH decreased immediately after the sucrose bath but increased again after 5 min.
We demonstrate here that the in vitro microbial model for the study of dentin caries formation is reproducible and able to produce dentin caries, irrespective
of the frequency of sucrose exposure. |
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