A packed-bed reactor utilizing porous resin enables high density culture of hepatocytes |
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Authors: | Kennichi Yanagi Hirotoshi Miyoshi Hideki Fukuda Norio Ohshima |
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Affiliation: | (1) Department of Biomedical Engineering, Institute of Basic Medical Sciences, University of Tsukuba, 1-1-1 Tennoudai, 305 Tsukuba City, Ibaraki, Japan;(2) Engineering Research Laboratories, Kaneka Corporation, 1-8 Miyamae, 676 Takasago, Hyogo, Japan |
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Abstract: | Summary To enable high density culture of hepatocytes for use as a hybrid artificial liver support system or a bioreactor system, a packed-bed reactor using collagen-coated reticulated polyvinyl formal (PVF) resin was applied to a primary culture of hepatocytes. Cubic PVF resins (2×2×2 mm, mean pore size: 100, 250 or 500 m) were used as supporting substrates to immobilize hepatocytes. Two hundred and fifty cubes were packed in a cylindrical column, and 2.6–11.3×107 hepatocytes were seeded in the column by irrigating with 3 ml of the medium containing hepatocytes. Perfusion culture experiments using this packed-bed reactor, as well as monolayer cultures using conventional collagen-coated petri dishes as control experiments, were performed. Sufficient amounts of hepatocytes were found to be immobilized in the reticulated structure of the PVF resins. The highest density of immobilized hepatocytes attained with PVF resin was 1.2×107 cells/cm3 PVF, which showed levels of ammonium removal and urea-N secretion comparable to those in the monolayer culture. It is concluded that the packed-bed reactor system utilizing PVF resin is a promising process for developing a bioreactor or a bioartificial organ using hepatocytes.Correspondence to: N. Ohshima |
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